DNA polymerase II
Class of enzymes / From Wikipedia, the free encyclopedia
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DNA polymerase II (also known as DNA Pol II or Pol II) is a prokaryotic DNA-dependent DNA polymerase encoded by the PolB gene.[1]
DNA Polymerase II | |||||||
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Identifiers | |||||||
Organism | Escherichia coli (str. K-12 substr. MG1655) | ||||||
Symbol | polB | ||||||
Entrez | 944779 | ||||||
PDB | 3K5M | ||||||
RefSeq (Prot) | NP_414602.1 | ||||||
UniProt | P21189 | ||||||
Other data | |||||||
EC number | 2.7.7.7 | ||||||
Chromosome | genome: 0.06 - 0.07 Mb | ||||||
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DNA Polymerase II is an 89.9-kDa protein and is a member of the B family of DNA polymerases. It was originally isolated by Thomas Kornberg in 1970, and characterized over the next few years.[2][3][4] The in vivo functionality of Pol II is under debate, yet consensus shows that Pol II is primarily involved as a backup enzyme in prokaryotic DNA replication. The enzyme has 5′→3′ DNA synthesis capability as well as 3′→5′ exonuclease proofreading activity. DNA Pol II interacts with multiple binding partners common with DNA Pol III in order to enhance its fidelity and processivity.[1]