Sucrose lysis test
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The sucrose lysis test is a diagnostic laboratory test used for diagnosing paroxysmal nocturnal hemoglobinuria (PNH), as well as for hypoplastic anemias and any hemolytic anemia with an unclear cause.[1] The test works by using sucrose, which creates a low ionic strength environment that allows complement to bind to red blood cells.[1] In individuals with PNH, some red blood cells are especially vulnerable to lysis caused by complement.[1] The test may also produce suspicious results in other hematologic conditions, including megaloblastic anemia and autoimmune hemolytic anemia.[1] False-negative results can occur when complement activity is absent in the serum.[1] A simpler alternative called the sugar water test also involves mixing blood with sugar and observing for hemolysis, using the same principle as the sucrose lysis test.[1]
Sucrose lysis test | |
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Synonyms | Sucrose hemolysis test |
Purpose | screening and diagnosis of paroxysmal nocturnal hemoglobinuria |