Schiff test
Organic chemistry named reaction / From Wikipedia, the free encyclopedia
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The Schiff test is an early organic chemistry named reaction developed by Hugo Schiff,[1] and is a relatively general chemical test for detection of many organic aldehydes that has also found use in the staining of biological tissues.[2] The Schiff reagent is the reaction product of a dye formulation such as fuchsin and sodium bisulfite; pararosaniline (which lacks an aromatic methyl group) and new fuchsin (which is uniformly mono-methylated ortho to the dye's amine functionalities) are not dye alternatives with comparable detection chemistry.
Classification | Colorimetric method |
---|---|
Analytes | Aldehydes |
In its use as a qualitative test for aldehydes, the unknown sample is added to the decolorized Schiff reagent; when aldehyde is present a characteristic magenta color develops. Schiff-type reagents are used for various biological tissue staining methods, e.g. Feulgen stain and periodic acid-Schiff stain. Human skin also contains aldehyde functional groups in the termini of saccharides and so is stained as well.